An improved assay for the spectrophotometric determination of chondroitinase ABC activity
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Effect of chondroitinase ABC on inflammatory and oxidative response following spinal cord injury
Objective(s): Chondroitinase ABC (cABC) treatment improves functional recovery following spinal cord injury (SCI) through degrading inhibitory molecules to axon growth. However, cABC involvement in other pathological processes contributing to SCI remains to be investigated. Here, we studied the effect of cABC I on oxidative stress and inflammation developed in a rat model of SCI.Materials and M...
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Background: Chondroitinase ABC (cABC), due to its loosening impact on the extracellular matrix scaffold, has been used to enhance regeneration of injured axonal tracts after spinal cord injury (SCI). However, cABC thermal instability at physiological temperature has limited its clinical application. The disaccharide trehalose has been shown to increase the stability of cABC in body temperature....
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An extractive spectrophotometric method is described for the assay of sitagliptin phosphate in bulk drug and in tablets. Sitagliptin phosphatewas extracted as an ion-pair complex from the solution containing Eriochrome black T and 0.1N HCl into chloroform and the absorbance of the complex was measured at 500nm.The working conditions of the method were investigated and optimized. Beer’s law plot...
متن کاملHigh performance liquid chromatographic assay of disaccharides and oligosaccharides produced by the digestion of glycosaminoglycans with chondroitin sulphate lyases.
In high performance liquid chromatographic procedures hitherto described, SiO2, NH2 and RP columns have been used for the analysis of disaccharides produced by the digestion of glycosaminoglycans with the chondroitin sulphate lyases AC and ABC. The use of a potent anion exchanger offers the following advantages over these columns: superior separation characteristics for non-sulphated disacchari...
متن کاملEffect of Chondroitinase ABC Enzyme on Glial Fibrillary Acidic Protein, Chondroitin Sulfated Proteoglycans and Chondroitin 4-Sulfate Levels in an Animal Model of Spinal Cord Injury
Background: Following spinal cord injury, reactive astrocytes upregulate chondroitin sulfate proteoglycans (CSPGs) which act as a barrier to neuronal repair and regeneration. Therefore, enzymatic digestion of CSPGs by chondroitinase ABC (cABC) is a key strategy in the treatment of spinal cord injury. Furthermore, cABC has been shown to attenuate post spinal cord injury inflamma...
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